Hepatocyte Asialoglycoprotein Receptor Assay Using Stable Isotopes and Neutron Activation Analysis
نویسندگان
چکیده
منابع مشابه
Identification and quantification of the rat hepatocyte asialoglycoprotein receptor.
The asialoglycoprotein receptor from rat liver was purified by solubilization and affinity chromatography on asialoorosomucoid-Sepharose. The preparation yielded four distinct polypeptides of Mr 40,000-120,000. We prepared a monoclonal antibody that both immunoprecipitates solubilized receptor activity and blocks the binding of galactose-terminal glycoproteins to immobilized receptor. The monoc...
متن کاملDistribution of an asialoglycoprotein receptor on rat hepatocyte cell surface
Direct ferritin immunoelectron microscopy was applied to visualize the distribution of the hepatocyte cell surface of the asialoglycoprotein receptor which is responsible for the rapid clearance of serum glycoproteins and lysosomal catabolism. For this purpose, rabbit antibody against the purified hepatic binding protein specific for asialoglycoproteins was prepared and coupled to ferritin by g...
متن کاملUsing Stable Isotopes
Introduction Direct measurement of de novo lipogenesis has not previously been possible in humans. We measured de novo hepatic lipogenesis in normal men by means of stable isotopes and by combining the acetylated-xenobiotic probe technique with mass isotopomer analysis of secreted very low density lipoproteinfatty acids (VLDL-FA). Sulfamethoxazole (SMX) was administered with ['3Clacetate during...
متن کاملNeutron Activation Analysis Neutron Activation Analysis
CONTENT NEUTRON ACTIVATION ANALYSIS
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Introduction: Nowadays, many people use medicinal plants to manage diseases; therefore, detailed knowledge of the type and level of elements present in these plants is of prominent importance.The present study aimed to determine the weight fraction of 12 elements in the five most common medicinal plants in Iran. The names of these plants are caraway (Carum carvi), savo...
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ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 2000
ISSN: 0009-9147,1530-8561
DOI: 10.1093/clinchem/46.9.1519